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论文
题目:Death rates at specific life stages mold the sex gap in life expectancy
网址: https://www.pnas.org/doi/full/10.1073/pnas.2010588118
代码网址
https://github.com/CPop-SDU/sex-gap-e0-pnas
该文章发表于2021年,论文中图形对我们一部分同学仍具参考价值。作者提供的全套的代码和数据,可以直接使用。此外,作者的数据和代码写的非常的规整。但是,需要看懂和运行代码,还是需要有一定的基础。
论文主图
论文主图仅有两张,如下图所示。
代码
Figure 1
# function to localize pathsdevtools::source_gist("32e9aa2a971c6d2682ea8d6af5eb5cde")# prepare session
source(lp("0-prepare-session.R"))# theme -------------------------------------------------------------------
load("../dat/palettes.rda" %>% lp)theme_custom <- theme_minimal(base_family = font_rc) +theme(legend.position = "bottom",strip.background = element_blank(),strip.text = element_blank(),panel.grid.minor = element_blank(),panel.grid.major = element_line(size = .25),panel.ontop = T)
作者将相关的代码编写在其他的R脚本中,使用时直接进行调用。
# Fig 1 -- RELATIVE ----------------------------------
load("../dat/a6gap33cntrs.rda" %>% lp)# relative
df6 %>% filter(country %>% is_in(c("SWE", "USA", "JPN", "RUS"))) %>%mutate(name = name %>% fct_recode(USA = "United States") %>% fct_rev()) %>%ggplot() +geom_col(aes(year, ctb_rel %>% multiply_by(100), fill = age_group),position = position_stack(reverse = TRUE),color = NA,width = 1) +facet_grid(name ~ ., scales = "free_y", space = "free") +coord_cartesian(ylim = c(-10, 120), expand = FALSE)+scale_x_continuous(breaks = seq(1800, 2000, 50))+scale_y_continuous(breaks = seq(0, 100, 25), position = "right")+scale_fill_manual(values = pal_six, guide = guide_legend(ncol = 1, reverse = TRUE)) +theme_minimal(base_family = font_rc, base_size = 20) +theme(legend.position = c(.6, .5),strip.background = element_blank(),strip.text = element_blank(),panel.grid.minor = element_blank(),panel.grid.major = element_line(size = .1),panel.spacing = unit(0, "lines"),panel.ontop = T)+labs(x = NULL,y = "Contribution, %",fill = "Age group")+# label countriesgeom_text(data = . %>% select(name, row, column) %>% distinct(),aes(label = name, color = name), x = 2015, y = 120, hjust = 1, vjust = 1, size = 9, fontface = 2,family = font_rc)+scale_color_manual(values = pal_four %>% rev, guide = FALSE)one_outer <- last_plot()
one_outer# plot ratio
load("../dat/df4qx.rda" %>% lp)df4qx %>%pivot_wider(names_from = sex, values_from = qx) %>% ggplot(aes(age, y = m/f, color = country))+geom_hline(yintercept = 1, color = "gray25", size = .5)+geom_smooth(se = F, size = 1, color = "#ffffff", span = .25)+geom_smooth(se = F, size = .5, span = .25)+scale_x_continuous(breaks = c(0, 15, 40, 60, 80))+scale_y_continuous(trans = "log", breaks = c(.5, 1, 2, 3), labels = c("", 1, 2, 3),limits = c(.75, 3.5))+scale_color_manual(NULL, values = pal_four)+theme_minimal(base_family = font_rc, base_size = 16)+theme(legend.position = "none",panel.grid.minor = element_blank())+labs(y = "Sex ratio, log scale",x = "Age")+annotate("text", x = 50, y = .9, label = "Most recent year",size = 8.5, color = "grey50", alpha = .5,vjust = 1, family = font_rc, fontface = 2)one_a <- last_plot()
one_a
# Death risk Ratio, Sweden, years 1750, 1800, 1850, 1900, 1960, 2019
# plot qx
load("../dat/qxdiff.rda" %>% lp)qxdiff %>% filter(country == "SWE", year %>% is_in(c(1800, 1900, 1960, 2019 ))) %>% ggplot(aes(age, y = ratio, color = year %>% factor))+geom_hline(yintercept = 1, color = "gray25", size = .5)+geom_smooth(se = F, size = .75, span = .4)+scale_x_continuous(breaks = c(0, 15, 40, 60, 80))+scale_y_continuous(trans = "log", breaks = c(.5, 1, 2, 3), labels = c("", 1, 2, 3),limits = c(.75, 3.5))+scale_color_viridis_d(end = .97)+theme_minimal(base_family = font_rc, base_size = 16)+theme(legend.position = c(.85, .75),legend.spacing.x = unit(.1, "line"),legend.key.height = unit(1, "line"),panel.grid.minor = element_blank())+labs(color = "Year",y = "Sex ratio, log scale",x = "Age")+annotate("text", x = 50, y = .9, label = "Sweden",size = 8.5, color = "#009C9C", vjust = 1, family = font_rc, fontface = 2)one_b <- last_plot()
one_b# plot difference
df4qx %>%pivot_wider(names_from = sex, values_from = qx) %>% ggplot(aes(x = age, y = m - f, color = country, group = country)) +geom_path(size = .5)+scale_color_manual(NULL, values = pal_four)+scale_x_continuous(breaks = c(0, 15, 40, 60, 80))+scale_y_continuous(trans = "log",breaks = c(.0001, .001, .01, .05),labels = c(.0001, .001, .01, .05) %>% paste %>% str_replace("0.", "."),limits = c(9e-6, .1))+theme_minimal(base_family = font_rc, base_size = 16)+theme(legend.position = c(.77, .25),legend.spacing.x = unit(.1, "line"),legend.key.height = unit(1, "line"),legend.text = element_text(size = 16),panel.grid.minor = element_blank())+labs(y = "Sex gap, log scale",x = "Age")one_c <- last_plot()
one_c# arrange and save
blank <- ggplot(tibble(x = 1, y = 1), aes(x, y))+geom_rect(xmin = -Inf, xmax = Inf,ymin = -Inf, ymax = Inf,fill = "#ffffff",color = NA)+theme_void()library(cowplot)
one <- ggdraw() +draw_plot(one_outer) +# white space for plotsdraw_plot(blank, x = 0, y = .75, width = 0.7, height = 0.25)+draw_plot(blank, x = 0, y = .55, width = 0.33, height = 0.42)+draw_plot(blank, x = 0, y = .33, width = 0.33, height = 0.67)+# inset plotsdraw_plot(one_a, x = 0, y = .66, width = .33, height = .33)+draw_plot(one_c, x = .34, y = .66, width = .33, height = .33)+draw_plot(one_b, x = 0, y = 0.35, width = .33, height = .33)+# annotate plot lettersdraw_text(LETTERS[c(1,3,2,4)], x = c(.01, .35, .01, .01),y = c(.99, .99, .66, .3), hjust = 0, vjust = 1, size = 20, family = font_rc, fontface = 2)ggsave(filename = "out/main-one.png" %>% lp, plot = one, width = 10, height = 10, type = "cairo-png"
)
**这样一连串的的就绘制出图1。但是,有多少同学可以知道作者绘制每个图形的数据类型是什么样呢?
**
如果大家有时间时间和精力可以可以试一下,如果不行,那么在本文的中点赞或留言,我们一起分开绘制每个图形,一起学习!!!!
附图
ENDING!!
往期文章:
1. 最全WGCNA教程(替换数据即可出全部结果与图形)
WGCNA分析 | 全流程分析代码 | 代码一
WGCNA分析 | 全流程分析代码 | 代码二
WGCNA分析 | 全流程代码分享 | 代码三
2. 精美图形绘制教程
精美图形绘制教程
小杜的生信筆記,主要发表或收录生物信息学的教程,以及基于R的分析和可视化(包括数据分析,图形绘制等);分享感兴趣的文献和学习资料!!
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