本文主要是介绍(附文献)HRP标记小鼠抗猴IgG二抗应用,希望对大家解决编程问题提供一定的参考价值,需要的开发者们随着小编来一起学习吧!
HRP(Horse radish Peroxidase,辣根过氧化物酶)是一种含亚铁血红素的蛋白质,广泛分布于植物界,它是由无色的酶蛋白和棕色的铁卟啉结合而成的糖蛋白,糖含量18%,分子量达44000 kDa。HRP易提取、价格低廉、性质稳定,耐热及有机溶剂,与抗原或一抗偶联后,活性很少受损失,在免疫学实验中广泛应用。
艾美捷/HRP标记小鼠抗猴IgG二抗概述:
克隆:SB108a
同型:小鼠(BALB/c)IgG1k
免疫原:Rhesus IgG
特异性:Rhesus and cynomolgus IgG
对人和兔免疫球蛋白、山羊IgG和小鼠、大鼠、仓鼠、豚鼠的最低反应性,绵羊、驴、牛、马、猪、猫和鸡血清。
艾美捷/HRP标记小鼠抗猴IgG二抗应用:
ELISA–质量检测1-8
FLISA–经过质量测试
FC——文献9报道
WB——文献10中有报道
HRP标记小鼠抗猴IgG二抗其他应用:由于应用不同,需要确定所使用产品的最/佳工作稀释度匹配需求。
HRP标记小鼠抗猴IgG二抗文献参考:
1. Kasturi SP, Skountzou I, Albrecht RA, Koutsonanos D, Hua T, Nakaya HI, et al. Programming the magnitude and persistence of antibody responseswith innate immunity. Nature. 2011;470:543-7. (ELISA)
2. Wang J, Tricoche N, Du L, Hunter M, Zhan B, Goud G, et al. The adjuvanticity of an O.volvulus-derived rOv-ASP-1 protein in mice using sequential vaccinations and in non-human primates. PLoS One. 2012;7(5):e37019. (ELISA)
3. Ferraro B, Talbott KT, Balakrishnan A, Cisper N, Morrow MP, Hutnick NA, et al. Inducing humoral and cellular responses to multiple sporozoite and liver-stage malaria antigens using exogenous plasmid DNA. Infect Immun. 2013;81:3709-20. (ELISA)
4. Padte NN, Boente-Carrera M, Andrews CD, McManus J, Grasperge BF, Gettie A, et al. A glycolipid adjuvant, 7DW8-5, enhances CD8+ T cell responses induced by an adenovirus-vectored malaria vaccine in non-human primates. PLoS One. 2013;8(10):e78407. (ELISA)
5. Sholukh AM, Watkins JD, Vyas HK, Gupta S, Lakhashe SK, Thorat S, et al. Defense-in-depth by mucosally administered anti-HIV dimeric IgA2 and systemic IgG1 mAbs: complete protection of rhesus monkeys from mucosal SHIV challenge. Vaccine. 2015;33:2086-95. (ELISA)
6. Bolton DL, Pegu A, Wang K, McGinnis K, Nason M, Foulds K, et al. Human immunodeficiency virus type 1 monoclonal antibodies suppress acute simian-human immunodeficiency virus viremia and limit seeding of cell-associated viral reservoirs. J Virol. 2015;90:1321-32. (ELISA)
7. Yang Z, Wang H, Salcedo TW, Suchard SJ, Xie JH, Schneeweis LA, et al. Integrated pharmacokinetic/pharmacodynamic analysis for determining the minimal anticipated biological effect level of a novel anti-CD28 receptor antagonist BMS-931699. J Pharmacol Exp Ther. 2015;355:506-15. (ELISA)
8. Kutzler MA, Wise MC, Hutnick NA, Moldoveanu Z, Hunter M, Reuter MA, et al. Chemokine-adjuvanted electroporated DNA vaccine induces substantial protection from simian immunodeficiency virus vaginal challenge. Mucosal Immunol. 2016;9:13-23. (ELISA)
9. Guo J, Zuo T, Cheng L, Wu X, Tang J, Sun C, et al. Simian immunodeficiency virus infection evades vaccine-elicited antibody responses to V2 region. J Acquir Immune Defic Syndr. 2015;68:502-10. (FC)
10. Parks CL, Koff WC, inventors; International AIDS Vaccine Initiative, assignee. Transgenic Vero-CD4-CCR5 cell line. United States patent applicaton publication US 2017/0266272 A1. 2017 Sep21. (WB)
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