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SouthernBiotech艾美捷山羊抗兔IgM FITC抗体,用于流式细胞术、免疫组织化学和免疫印迹分析。
SouthernBiotech 山羊抗兔IgM-FITC基本参数:
同型:山羊IgG
同型对照:山羊IgG-FITC
特异性:与兔IgM重链反应
来源:兔IgM高免疫山羊的混合抗血清
交叉吸附:兔IgG;可能与其他物种的免疫球蛋白发生反应
纯化方法:琼脂糖共价连接的混合兔IgM亲和层析
结合:荧光素
缓冲液配方:含有<0.1% die氮化钠的磷酸盐缓冲盐水
克隆性:多克隆
浓度:1.0毫克/毫升
体积:1.0毫升
推荐存储:2-8摄氏度;避免暴露在阳光下
应用:经过质量测试的相关格式
应用程序包括-ELISA、FLISA
工作稀释液:
ELISA:
AP conjugate 1:2,000 – 1:4,000
HRP conjugate 1:4,000 – 1:8,000
BIOT conjugate 1:5,000 – 1:20,000
FLISA:
FITC, TRITC, and AF555 conjugates 1:100 – 1:400
PE and AF647 conjugates ≤ 1 µg/mL
SouthernBiotech山羊IgG-TRITC文献引用:
1. Holdsworth RJ, McKenzie H, Parratt D, Irving AD, Cuschieri A. The role of the spleen in the immune response following naturally acquired exposure to encapsulated bacteria. Int J Exp Pathol. 1990;71:835-43. (ELISA)
2. Taylor-Robinson AW, Phillips RS. B cells are required for the switch from Th1- to Th2-regulated immune responses to Plasmodium chabaudi chabaudi infection. Infect Immun. 1994;62:2490-98. (ELISA)
3. Yamashita T, Freigang S, Eberle C, Pattison J, Gupta S, Napoli C, et al. Maternal immunization programs postnatal immune responses and reduces atherosclerosis in offspring. Circ Res. 2006;99:e51-64. (ELISA, ELISPOT)
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